Draft genome of Patiria pectinifera and its genome annotation and functional annotation information

Genomic DNA was extracted from the testis of Patiria pectinifera, collected at the Misaki Marine Biological Station, using standard protocols. DNA quality and quantity were assessed using a Qubit Fluorometer and a NanoDrop spectrophotometer. Illumina paired-end libraries with an insert size of approximately 500 bp were prepared using the TruSeq DNA Sample Preparation Kit (Illumina). Mate-pair libraries with insert sizes of approximately 3 kb, 6 kb, 10 kb, and 15 kb were constructed using the Nextera Mate Pair Sample Preparation Kit (Illumina). Sequencing was performed on an Illumina HiSeq 2500 platform, generating 2 × 250 bp paired-end reads for paired-end libraries and 2 × 100 bp paired-end reads for mate-pair libraries. Genome assembly was performed using Platanus-allee v2.2.1 , followed by scaffolding with mate-pair libraries and gap closing using paired-end reads. RNA-seq was performed on brachiolaria larvae and five metamorphic stages. Sequencing reads were mapped to the Patiria pectinifera draft genome using STAR (v2.7.3a). Strand-specific transcriptome assembly was conducted using Scallop (v0.10.4), and functional annotations for the assembled transcriptome were obtained using Trinotate (v3.1.0).本研究从三崎海洋生物站采集的多棘海盘车（Patiria pectinifera）精巢中提取基因组DNA，实验操作遵循标准流程。采用Qubit荧光计（Qubit Fluorometer）与NanoDrop分光光度计（NanoDrop spectrophotometer）评估DNA的质量与浓度。使用TruSeq DNA样本制备试剂盒（TruSeq DNA Sample Preparation Kit，Illumina）构建插入片段长度约500 bp的Illumina双端文库；使用Nextera Mate Pair样本制备试剂盒（Nextera Mate Pair Sample Preparation Kit，Illumina）构建插入片段长度分别约3 kb、6 kb、10 kb及15 kb的mate-pair文库。测序在Illumina HiSeq 2500平台上完成，双端文库生成2×250 bp双端读段，mate-pair文库则生成2×100 bp双端读段。基因组组装采用Platanus-allee v2.2.1完成，随后借助mate-pair文库进行基因组支架构建，并利用双端读段填补序列缺口。 对多棘海盘车的短腕幼虫（brachiolaria larvae）及5个变态发育阶段样本开展RNA测序（RNA-seq）。使用STAR (v2.7.3a) 将测序读段比对至多棘海盘车草图基因组。采用Scallop (v0.10.4) 进行链特异性转录组组装，随后通过Trinotate (v3.1.0) 为组装得到的转录组获取功能注释信息。