Transcriptional profiling of cochlear spiral ganglion neurons and lateral wall cells

In order to characterize the response to noise of various cell types in the inner ear, the cochleae from wild-type mice, naive or subjected to a 2h, 105 dB SPL noise insult, were dissected out. To further examine spiral ganglion neurons in more detail, non-neuronal populations were removed in one set of samples. In another set of samples, the lateral wall tissue was isolated to better understand how cells from the stria respond to noise insults. The resulting tissue was then dissociated to obtain a single cell suspension, and profiled by single cell RNA-seq. 4 naïve and 4 noise-exposed samples (biological replicates) were profiled for each of the neuronal- and lateral wall-focused dissections, for a total of 16 samples.