Characterization and MicroRNA Levels of Serum-Derived Extracellular Vesicles in Hepatitis B Virus-Related diseases

Serum samples were collected from patients with CHB, DeCi and normal controls. We separated EVs from serum and identified them. MiRNAs from EVs were sequenced by NGS and analyzed to obtain differentially expressed miRNAs. Then we detected the expression level of candidate miRNAs from every single sample by qRT-PCR. The differences of serum EVs in patients with liver disease and 4 miRNAs with significantly different expression levels in EVs were determined. These serum EVs and miRNAs in EVs may be candidates for diagnosing and monitoring liver disease, or important therapeutic targets for the treatment of liver disease. Overall design: We collected Serum samples from patients with CHB, DeCi and normal controls. Every 5 samples of the same group are randomly mixed into a pool. EVs RNAs were extracted from 5 pooled CHB serum samples, 5 pooled DeCi serum samples and 5 pooled healthy serum samples. MiRNAs were sequenced by NGS and analyzed to obtain differentially expressed miRNAs.