circRNA Microarray analysis of hippocampus from mouse treated by HFD

To explore the potential mechanism linking circRNAs in Obesity-associated cognitive impairment induced by a longtime high-fat diet. The male mouse was treated by HFD up to16 weeks . Then the hippocaum tissues was isloated and circRNA Microarray analysis was applied. The expression profiles of hippocaum from mouse treated with HFD changed and 2 circRNA (mmu-circRNA-004797,mmu-circRNA-21040) from this signature was quantified by real-time PCR, confirming high-fat diet can change the profiles of circRNAs of hippocaum from mouse and may participated in the obesity associated cognitive impairment. 6-week-old mice were randomly divided into two groups, fed with standard diet (STD) and high-fat diet(HFD), respectively. Hippocampal tissue was isolated for circRNA detection after 16 weeks of high-fat feeding