Generation of ventralized thalamic organoids with inhibitory thalamic reticular nucleus. Generation of ventralized thalamic organoids with inhibitory thalamic reticular nucleus

Single-cell RNA-seq was performed in SHH-treated thalamic organoids Overall design: Human thalamic organoids (ThOs) resembling the dorsal thalamus by dual SMAD inhibition and insulin-driven caudalization coupled with thalamic patterning (MEK-ERK inhibition and BMP7 activation) from hESCs. The organoids were further guided into ventral identity with SHH treatment.