Single-cell RNA-Sequencing of splenocytes from Four Core Genotype (FCG) mice

This repository contains single-cell gene expression profiling from mouse splenocytes of the Four Core Genotypes cross. By crossing mice with both a deletion of the sex-determining factor Sry on the Y-chromosome and a transgenic insertion of Sry on Chromosome 3 , four combinations of gonadal (testis or ovaries) and chromosomal (XX or XY) are generated, namely XYSry-Chr3Sry+ (gonadal and chromosomal males), XYSry- (gonadal females, chromosomal males), XX (gonadal and chromosomal females), XXChr3Sry+ (gonadal males, chromosomal females). The transgenes were on a C57BL6 genetic background which is crossed with a CAST/EiJ female to allow for the distinction of the parental haplotypes. From all four genotypes, splenocytes are isolated and subjected to 10x Genomics single-cell RNA-Sequencing.