Rana pretiosa Genome sequencing and assembly. Rana pretiosa

Retaining sufficient genetic variability for both short and longterm sustainability is a chief aim of ex situ programs for threatened species. Conservation breeding and reintroduction programs exist but oftentimes little is known about the genetic health of in situ or ex situ populations. We collected genetic samples from both wild and zoo populations of the endangered Oregon Spotted Frog and compared their genetic diversity using single nucleotide polymorphisms. We also calculated inbreeding coefficients and effective population sizes and assessed population structure to inform current breeding strategies. The patterns we uncovered support continued collaboration of ex situ and in situ endeavours as supplementation will likely be required for the longterm viability of the very wild populations the zoos rely on for genetic sustainability.