Transcriptome profiling of pathological cardiac hypertrophy

Here, we performed pathological cardiac hypertrophy transcriptome analyses by using heart tissue from Sham or TAC surgery to identify the charecteric in pathological cardiac hypertrophy. In addition, through combined analysis with the aging-associated transcriptome to identify the similarity and difference between heart ageing and pathological cardiac hypertrophy. Wild-type male mice at 2-month of age were raised in a stable environment (room temperature, 24 ± 3 °C; room humidity, 55 ± 5%) with a 12-h light/12-h dark cycle, and were fed normal chow, were randomly separated into sham and TAC groups, with at least 6 mice per group. Transverse aortic constriction (TAC) was performed as previously described with 27G needle. The mice were anaesthetized with inhalational anaesthetic and euthanized by cervical dislocation. Hearts were immediately dissected and stored at -80°C. Three independent biological replicate samples from Sham or TAC group were used for RNA extraction. RNA-seq was completed by Beijing Genomics Institution (BGI).