RNA-seq of Monkeypox virus-protein overexpression in human embryonic stem cells (hESCs) revealed the effects of H3L, A29L, A35R and I1L in human

Monkeypox virus (MPV) can infect human cells and induces injuries in human. However, whether and how MPV affect the function of human cells remain to be elucidated. Here, we overexpressed H3L, A29L, A35R and I1L in hESCs, separately. We appliced RNA-seq on these hESCs and want to know gene expression patterns of control and Overexpression hESCs. Subsequent data analyses will help us to know whether and how H3L, A29L, A35R or I1L affect cells in human. Overall design: We separately cloned DNA seqeunces of H3L, A29L, A35R and I1L into lentivirus and infected hESCs, follwed with puro screening. The stable cell lines were rotinely maintained in mTesR1 medium in 6-well plate. Stable cell lines were collected for RNA purification and RNA-seq. Lenti-virus control cell line (control hESCs) did not express any protein. Total RNAs were extracted and purified with RNeasy Mini Kit (Qiagen). 4 biological replicates were applied for each overexpression cell line.