Figure S1 - Bothrops jararaca Venom Metalloproteinases Are Essential for Coagulopathy and Increase Plasma Tissue Factor Levels during Envenomation

Platelet counts (a), and plasma fibrinogen levels (b) in rats 3 h after BjV administration. Rats were injected s.c. (1.6 mg/kg) with venom incubated with saline (BjV+Saline), 13 mM Na2-EDTA (BjV+Na2-EDTA), 4 mM AEBSF (BjV+AEBSF), 13 mM 1,10 phenathroline (BjV+o-phe) or ethanol (BjV+Ethanol, control group for BjV+o-phe). Control animals were treated under the same conditions, but were injected with saline (Saline). *pSaline). #pBjV+Saline. ⧫A statistically significant difference was noticed between BjV+Na2-EDTA and BjV+o-phe for plasma fibrinogen (p = 0.005). Data are expressed as mean ± s.e.m (n = 5–6/group). (EPS)