TGF-beta1 time-course in A-549 and MDA-231-D cell lines profiled with CAGE-seq

A-549 Lung cancer cells and MDA-231-D highly metastatic human breast cancer cells were treated with TGF-beta1 at the final concentration of 1mg/mL. At 0, 1, 2, 4, 6, and 24 hours post stimulation, cells were harvested followed by RNA extraction using RNeasy mini kit. Transcriptome data was produced by nAnT-iCAGE. CAGE libraries were sequenced on Illumina HiSeq 2500 (50-nt single read).