Contribution of VEGF-B-induced endocardial endothelial cell lineage in physiological versus pathological cardiac hypertrophy.

ScRNA-seq was used to investigate the effects of autocrine versus paracrine VEGF-B signaling in the heart using transgenic mouse models. The paracrine model was further investigated in pregnancy-induced cardiac hypertrophy as well as in mice with ligation of the left anterior descending (LAD) coronary artery. Overall design: Murine hearts were isolated and digested followed by analysis of the stromovascular fraction (SVF) by scRNA-seq, or FAC sorting of the cardiac enodthelial cells (ECs) by using FITC-tagged Pecam1 antibody and analysis by scRNA-seq.