Genes downregulated by miR-92a-1-5p overexpression in K562 cells treated with imatinib.

In order to indetify new target genes of miR-92a-1-5p in chronic myeloid leukemia, K562 cells carrying the BCR::ABL1 fusion gene were transiently transfected with miR-92a mimic or scramble and treated with imatinib. RNA was sequenced to identify genes expressed under imatinib treatment and downregulated after miR-92a-1-5p overexpression. Overall design: K562 cells (5 million) were nucleoporated using Amaxa Nucleofector Technology (Lonza) with 500 pmol of hsa-miR-92a-1-5p synthetic miRVana mimic (#4464066) or miRVana miRNA Mimic, Negative Control #1(#4464058) (Thermo Fisher Scientific). 4h after transfection, cells were treated with 1 µM of Imatinib. 24h later, RNA was extracted and sent to Macrogen for RNA- sequencing. A paired-end sequencing was performed with an average sequencing coverage of 62 M. Results obtained were quantified using the Kallisto software.