Comparison of SPRi and BLI technologies with respect to throughput and sample consumption in performing a comprehensive classical sandwich epitope binning assay on 96

* Robotic integration with the BLI platform results in a higher unattended throughput, longer unattended runtime, and significantly shorter total runtime by automating multi-plate analyses. Less time is needed for sample preparation if a robot is used for the microfluidic dispensing.
**Sample volumes can be reduced by 50% if tilted-bottom 384-well microplates that hold a minimum of 40 μl/well are used instead of the standard flat-bottom 384-well microplates.