Four ITS reference sequence databases (D1–D4) used for the training of the QIIME2 q2-feature-classifier

Four ITS reference sequence databases (D1–D4) were used to train the QIIME2 q2-feature-classifier. Database D1 includes 196,344 fungal ITS reference sequences from the Unite database37, while the D2 database contains 32,013 macrofungi ITS reference sequences from the Unite database. Database D3 contains all reference sequences of the D2 database and 189 macrofungi ITS reference sequences from GenBank (see Table S4 for detailed information). Finally, Database D4 includes all reference sequences of the D3 database and 5,733 macrofungi ITS reference sequences from the RefSeq database (https://www.ncbi.nlm.nih.gov/refseq/). Four QIIME2 q2-feature-classifier datasets (C1–C4) were generated from the four databases (D1-D4) using the fit-classifier-naive-bayes option of QIIME 2 (v2022.2)