Chan-Soo Lee, Chang-Ki Choi, Eun-Young Shin, Martin Alexander Schwartz, Eung-Gook Kim (2011) CIL:26532, Mus musculus, fibroblast. CIL. Dataset

To study the molecular mechanism by which nonmuscle myosin II (MII) regulates protrusion and adhesion dynamics in migrating cells, NIH3T3 cells were transfected with myc-tagged phosphomimetic constitutively active MLC mutants (MLCee, green). After 20' exposure to PDGF, which induces myosin-inactivation, stress fiber disassembly, and stimulates fibroblast motility, the cells were fixed and co-stained for TRIO (a multifunctional, multidomain Rho guanine nucleotide exchange factor, red) and filamentous actin (blue). These findings help elucidate a functional link between MII and Rac1/Cdc42 GTPases, which may regulate protrusion/adhesion dynamics in migrating cells. This image is original data from Fig. S5 "Expression of active MLC mutants inhibits the dissociation of MII–Trio complex," in J. Cell Biol. 2010. Vol. 190(4):663–674

本研究旨在探究非肌肉肌球蛋白II（MII）调节迁移细胞突起和粘附动力学的作用分子机制。通过将标记有myc标签的磷模拟型恒定活性MLC突变体（MLCee，绿色）转染NIH3T3细胞，暴露于诱导肌球蛋白失活、应力纤维解聚并刺激成纤维细胞运动的PDGF（20分钟）后，细胞被固定并进行TRIO（一种多功能、多结构域的Rho鸟苷酸交换因子，红色）和丝状肌动蛋白（蓝色）的共染色。这些发现有助于阐明MII与Rac1/Cdc42 GTP酶之间的功能联系，这些酶可能调节迁移细胞中的突起/粘附动力学。本图像为J. Cell Biol. 2010年，第190卷第4期第663-674页图S5 "活性MLC突变体的表达抑制MII-Trio复合物的解离"中的原始数据。