Stress-related transcriptomic changes associated with GFP transgene overexpression and active transgene silencing in plants

We performed gene expression analysis using RNA-seq data of four plant samples for each of the following groups: Wild Type (WT) plants and transgenic GFP6.4 Non-Silenced (NS), Ongoing Spreading (OS) post-transcriptional gene silencing, and Systemically Spread (SS) post-transcriptional gene silencing. The analysis reveals that spreading and maintenance of GFP-PTGS are associated with transcriptional reprogramming of genes that are involved in abiotic stress responses and pattern- or effector-triggered immunity-based stress responses.