Comparison of RNA target abundances in Illumina sequence reads compared with qPCR results.
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The expected log2 differential is taken as the log2 of fold change in CCD colonies from Table 2. For read counts, differential abundance = log2 (CCD+ reads) – log2 (CCD− reads) – log2 (total CCD+ reads/total CCD− reads). N/A indicates that the log2 difference in abundance could not be calculated because no reads matched in CCD−.
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2015-12-02
