Transcriptomics profiling of gonadal white adipose tissue (gWAT) of wild-type (WT) and macrophage-specific Bnip3 knockout (MKO) mice fed a normal chow diet (NCD) or high-fat diet (HFD)

Obesity induces phenotypic changes and pro-inflammatory polarization of adipose tissue macrophages, contributing to chronic inflammation and insulin resistance. This study investigates the influence of Bnip3 on adipose tissue inflammation and the metabolic dysfunction prompted by a high-fat diet. Our objective involves examining the impact of a 12-week HFD feeding on both WT and Bnip3 MKO mice, comparing the differentially regulated genes based on dietary intake. Overall design: Deep sequencing generated the gWAT mRNA profiles of WT and Bnip3 MKO mice fed a NCD or HFD. Three biological replicates were analyzed for each condition.