Antimicrobial, Probiotic, and Immunomodulatory Potential of Cannabis sativa Extract and Delivery Systems

Article, Dataset for the article Abstract The compounds present in hemp show multidirectional biological activity. It is related to the presence of secondary metabolites, mainly cannabinoids, terpenes, and flavonoids, and the synergy of their biological activity. The aim of this study was to assess the activity of the Henola Cannabis sativae extract and its combinations with selected carriers (polyvinyl caprolactam–polyvinyl acetate–polyethylene glycol graft copolymer, magnesium aluminometasilicate, and hydroxypropyl-β-cyclodextrin) in terms of antimicrobial, probiotic, and immunobiological effects. As a result of the conducted research, the antimicrobial activity of the extract was confirmed in relation to the following microorganisms: Clostridium difficile, Listeria monocytogenes, Enterococcus faecalis, Staphylococcus aureus, Staphylococcus pyrogenes, Escherichia coli, Klebsiella pneumoniae, Salmonella typhimurium, Pseudomonas aereuginosa, and Candida albicans (microorganism count was reduced from ~102 CFU mL−1 to <10 CFU mL−1 in most cases). Additionally, for the system with hydroxypropyl-β-cyclodextrin, a significant probiotic potential against bacterial strains was established for strains Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus rhamnosus, Lactobacillus reuteri, Pediococcus pentosaceus, Lactococcus lactis, Lactobacillus fermentum, and Streptococcus thermophilus (microorganism count was increased from ~102 to 104–107). In terms of immunomodulatory properties, it was determined that the tested extract and the systems caused changes in IL-6, IL-8, and TNF-α levels. Keywords: Cannabis sativa; cannabidiol; antibacterial; probiotic; immunomodulatory; immunostimulatory论文与配套数据集 摘要 大麻中所含的多种成分展现出多向生物学活性，这与其含有的次生代谢产物密切相关，主要包括大麻素类、萜类及黄酮类物质，且各类成分的生物活性具有协同效应。本研究旨在评估Henola大麻（Cannabis sativae）提取物及其与选定载体的复合体系的活性，所选载体包括聚乙烯己内酰胺-聚乙酸乙烯酯-聚乙二醇接枝共聚物（polyvinyl caprolactam–polyvinyl acetate–polyethylene glycol graft copolymer）、铝硅酸镁（magnesium aluminometasilicate）以及羟丙基-β-环糊精（hydroxypropyl-β-cyclodextrin），考察其抗菌、益生及免疫生物学效应。 本研究证实该提取物对下述微生物具有抗菌活性：艰难梭菌（Clostridium difficile）、单核细胞增生李斯特菌（Listeria monocytogenes）、粪肠球菌（Enterococcus faecalis）、金黄色葡萄球菌（Staphylococcus aureus）、化脓葡萄球菌（Staphylococcus pyrogenes）、大肠埃希菌（Escherichia coli）、肺炎克雷伯菌（Klebsiella pneumoniae）、鼠伤寒沙门菌（Salmonella typhimurium）、铜绿假单胞菌（Pseudomonas aeruginosa）及白色念珠菌（Candida albicans）；多数情况下可将微生物菌落数从约10² CFU·mL⁻¹降至10 CFU·mL⁻¹以下。 此外，搭载羟丙基-β-环糊精的复合体系对下述菌株展现出显著的益生潜力：嗜酸乳杆菌（Lactobacillus acidophilus）、干酪乳杆菌（Lactobacillus casei）、植物乳杆菌（Lactobacillus plantarum）、短乳杆菌（Lactobacillus brevis）、鼠李糖乳杆菌（Lactobacillus rhamnosus）、罗伊氏乳杆菌（Lactobacillus reuteri）、戊糖片球菌（Pediococcus pentosaceus）、乳酸乳球菌（Lactococcus lactis）、发酵乳杆菌（Lactobacillus fermentum）及嗜热链球菌（Streptococcus thermophilus）；可将微生物菌落数从约10²提升至10⁴~10⁷。 在免疫调节特性方面，本研究证实受试提取物及其复合体系可改变IL-6、IL-8及TNF-α的表达水平。 关键词：大麻（Cannabis sativa）；大麻二酚（cannabidiol）；抗菌；益生；免疫调节；免疫刺激