Adhesion of Marinobacter hydrocarbonoclasticus to surfactant-decorated oil droplets

This project investigated the effect of surfactant chemistry and concentration on the initial adhesion of Marinobacter hydrocarbonoclasticus at the interface of dodecane droplets suspended in water, investigating both the time-dependent adhesion of bacteria at the dodecane/water interface and the dependence of adhesion on droplet size and surfactant concentration. The dataset consists of images (.bmp) for dodecane-in-water emulsions prepared using co-flow microfluidic device, z-stack images (.tiff) for three different drop sizes at different time intervals, z-stack images (.tiff) for two different drop sizes at different surfactant concentrations and pendant drop movies (.avi files) at different surfactant concentrations for the dodecane/water system. The data analyzed consists of: (1) normalized drop diameter as a function of inner to outer fluid flow rates in microfluidic device, (2) number of cell and cells normalized with drop surface area as a function of time, (3) time constant of adhesion as a function of drop diameter, (4) orientation distribution of cells at dodecane/water interface for three drop sizes, (5) cell areal density as a function of surfactant concentration, (6) interfacial tension as a function of surfactant concentration, and (7) cell areal density as a function of interfacial tension.

本研究考察了表面活性剂化学性质与浓度对水相悬浮十二烷液滴界面上解烃海杆菌（Marinobacter hydrocarbonoclasticus）初始黏附行为的影响，同时探究了细菌在十二烷/水界面的黏附随时间的变化规律，以及黏附行为对液滴尺寸与表面活性剂浓度的依赖性。本数据集包含以下内容：采用同轴流微流控装置制备的水包十二烷乳液的位图（.bmp）图像、三种不同液滴尺寸在不同时间区间下的Z轴堆叠（Z-stack）图像（.tiff）、两种不同液滴尺寸在不同表面活性剂浓度下的Z轴堆叠（Z-stack）图像（.tiff），以及十二烷/水体系在不同表面活性剂浓度下的悬滴视频（.avi文件）。所分析的数据涵盖：(1) 微流控装置内外流体流量比与归一化液滴直径的函数关系，(2) 细胞数量及以液滴表面积归一化的细胞数随时间的变化关系，(3) 黏附时间常数随液滴直径的变化关系，(4) 三种液滴尺寸下十二烷/水界面处细菌细胞的取向分布，(5) 细胞面密度随表面活性剂浓度的变化关系，(6) 界面张力随表面活性剂浓度的变化关系，(7) 细胞面密度随界面张力的变化关系。