Screening for resistance against the banana aphid Pentalonia nigronervosa (Hemiptera: Aphididae) and Banana bunchy top virus (genus, Babuvirus) in Musa genotypes in Central Africa.

Banana and plantain (Musa spp) represent an important food and cash crop worldwide. Their cultivation faces many pest and disease constraints amongst which banana bunchy top disease (BBTD). Banana bunchy top virus (BBTV, genus Babuvirus), the causal agent of BBTD is transmitted persistently by the banana aphid Pentalonia nigronervosa Coquerel (Hemiptera: Aphididae). Information regarding aphid’s preference to Musa genotypes is yet scanty whereas a high level of resistance to BBTD is not yet found. This study was undertaken to assess the susceptibility of different Musa genotypes from different genomic composition to banana aphid and BBTV. Experimental infestation with banana aphid was done on 38 Musa genotypes in screenhouse while 44 Musa genotypes were planted in BBTD affected area and assessed for 36 months under natural infestation and infection by the aphid vector. Results showed significant differences in the performance of banana aphid between Musa genotypes. In the screenhouse, the diploid Calcutta 4 (AA) and Ney Poovan (AB) were the least suitable to aphid growth while AAB triploids (Waema, Ebang) were the most suitable to aphid growth. In natural conditions, AAB genotypes (Batard, Ebang, Essong, Elat) and AAAB tetraploid hybrids (CRBP 39, CRBP 969), were highly suitable to banana aphid and susceptible to BBTD. Meantime, AA diploids (Tapo, Pisang Tongat, Figue Sucree), the triploids Yangambi Km 5 and FHIA 25 were less suitable banana aphid and highly susceptible to BBTD with 100.0 % infection observed on Tapo. The tetraploid Balonkawe (ABBB), the AAB triloid PITA 21, the wild diploids Calcutta 4 (AA) and Balbisiana Los Banos (BB) escaped to BBTV infection at 36 MAP. Knowledge of genotype response to banana aphid and BBTD is useful in the selection of promising lines towards breeding varieties with resistance to BBTD.

香蕉与大蕉（*Musa* spp.，芭蕉属）是全球范围内重要的粮食与经济作物。其种植过程面临多种病虫害威胁，其中香蕉束顶病（Banana Bunchy Top Disease, BBTD）尤为严重。引发香蕉束顶病的病原为香蕉束顶病毒（Banana Bunchy Top Virus, BBTV，Babuvirus属），该病毒可通过香蕉蚜（*Pentalonia nigronervosa* Coquerel，半翅目：蚜科）进行持久性传毒。目前关于香蕉蚜对芭蕉属基因型的偏好性研究尚且匮乏，且尚未发现对香蕉束顶病具有高抗性的材料。本研究旨在评估不同基因组构成的芭蕉属基因型对香蕉蚜及香蕉束顶病毒的感病性。试验通过两种方式开展：一是在防虫网室中对38份芭蕉属基因型进行人工香蕉蚜接种侵染；二是将44份芭蕉属基因型种植于香蕉束顶病发生田块，在自然蚜虫传毒侵染条件下开展为期36个月的调查评估。试验结果表明，不同芭蕉属基因型对香蕉蚜的适合性存在显著差异。防虫网室试验中，二倍体材料‘Calcutta 4（AA型）’与‘Ney Poovan（AB型）’最不适于香蕉蚜生长，而AAB型三倍体材料‘Waema’、‘Ebang’则最适于香蕉蚜繁殖。自然田间条件下，AAB型基因型（Batard、Ebang、Essong、Elat）以及AAAB型四倍体杂交种（CRBP 39、CRBP 969）对香蕉蚜的适合性极高，且对香蕉束顶病易感。与此同时，AA型二倍体材料（Tapo、Pisang Tongat、Figue Sucree）以及三倍体材料‘Yangambi Km 5’与‘FHIA 25’对香蕉蚜的适合性较低，但对香蕉束顶病表现为高感，其中‘Tapo’的发病率达100.0%。四倍体材料‘Balonkawe（ABBB型）’、AAB型三倍体‘PITA 21’以及野生二倍体材料‘Calcutta 4（AA型）’与‘Balbisiana Los Banos（BB型）’在种植36个月（36 MAP，Months After Planting）时未感染香蕉束顶病毒。明确不同芭蕉属基因型对香蕉蚜及香蕉束顶病的响应特性，可为选育抗香蕉束顶病的优良品种提供理论依据与材料支撑。