Transcriptome analysis between wild-type and DM1-affected fibroblast.

Human skin fibroblast cell lines from 3 normal individuals and 3 individuals with DM1 in one pedigree were obtained from the biobank of Shanghai East Hospital. Human skin fibroblast cells were grown in DMEM (Life Technologies), NaPyr, GlutaMAX, ß-mercaptoethanol and 10% foetal bovine serum (FBS) supplemented medium as previously described (Richner et al. 2015). Cells were collected after 80% confluency with TRIzol reagent and stored at -80? for RNA-seq and RT-PCR. Overall design: Comparative gene expression profiling analysis of RNA-seq data for wild-type and DM1-affected fibroblast.