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Stable isotope ratio and elemental contents for salmon muscle tissue collected from the F/V Northwest Explorer during the 2022 International Year of the Salmon Pan-Pacific Winter High Seas Expedition@en

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DataONE2023-09-15 更新2024-06-08 收录
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North Pacific salmon (Oncorhynchus spp.) were caught by deploying trawl nets in April (4-18) 2022 during the winter expeditions of the International Year of the Salmon to the North Pacific high seas onboard the F/V Northwest Explorer. Trawl nets were deployed at each station in the top 50 meters of the water column and towed for 60 minutes (night) or 90 minutes (day) at 4 to 5 knots. A 2x2 cm sample of salmon muscle tissue was collected from above the lateral line and in front of the dorsal fin and stored at -80°C. Muscle samples were then processed in the laboratory at the University of British Columbia. Tissue was lyophilized in freeze drier for 48 hours, homogenized using mortar and pestle, encapsulated in tin (Sn) capsules, and sent for measurements of carbon and nitrogen stable isotope ratios and elemental contents at an external lab. Samples were analysed on an Elemental Analyser coupled to an Isotope Ratio Mass Spectrometer. Details on analytical procedure are provided in the supplementary materials 1 and 2. The data reported includes each sample ẟ13C, %C, ẟ15N, % N, and CN ratios, in addition to the metadata associated with each sample (e.g., coordinates of oceanographic station and bottom depth where salmon specimen was collected, etc.).

2022年4月4日至18日,科研人员搭乘捕捞渔船(F/V Northwest Explorer)执行“国际鲑鱼年”(International Year of the Salmon)北太平洋公海冬季科考航次,在此期间通过布设拖网采集到北太平洋鲑属(Oncorhynchus spp.)鱼类样本。本次航次在每个采样站位均于水体上层50米处布设拖网,以4至5节的航速拖曳网具,夜间拖曳时长为60分钟,日间则为90分钟。研究人员从每尾鲑鱼的侧线上方、背鳍前方采集尺寸为2×2厘米的肌肉组织样本,将其置于-80℃低温环境中保存。随后样本被运送至英属哥伦比亚大学(University of British Columbia)的实验室进行预处理:先将组织样本置于冷冻干燥机中冻干48小时,再用研钵和研杵进行均质化处理,之后将处理后的样品封装入锡(Sn)胶囊,送至外部实验室开展碳、氮稳定同位素比值与元素含量测定。样本检测采用元素分析仪(Elemental Analyser)与同位素比率质谱仪(Isotope Ratio Mass Spectrometer)联用系统完成。分析流程的详细技术细节详见补充材料1与补充材料2。本数据集涵盖每份样本的δ¹³C值、总碳百分含量(%C)、δ¹⁵N值、总氮百分含量(%N)及碳氮比(CN比值),同时附带每份样本对应的元数据,包括鲑鱼样本采集所在的海洋学站位坐标、底层水深等相关信息。
创建时间:
2023-10-31
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