Optode performance data associated with: Metabolic Multireactor: practical considerations for using simple oxygen sensing optodes for high-throughput batch reactor metabolism experiments

This data package is associated with the publication “Metabolic Multireactor: practical considerations for using simple oxygen sensing optodes for high-throughput batch reactor metabolism experiments”, submitted to PlosONE (Kaufman et al. 2023; 10.1101/2023.03.28.534656). We carried out many testing and calibration experiments on a system of small oxygen consumption batch reactors designed for use with water and sediment samples for environmental questions. The oxygen sensing system is based very directly on the work of Larsen, et al. [2011], and similar oxygen sensing technology is widely used in the literature. Our primary focus was on practical considerations, such as temperature effects, lighting angle effects, sterilization, and other similar situations that a user may find useful. Most of the tests required comparing “base” calibration curves to “treatment” calibration curves to determine the extent to which the treatment impacted the reported measurements. This data package contains the performance and calibration data collected for that purpose. This dataset is comprised of one data folder containing (1) file-level metadata; (2) data dictionary; (3) readme; (4) diffusion test result files; (5) limit of detection test result files; (6) temperature impact files; (7) a main data file that contains test results for all other tests; and (8) an R script that uses Kolmogorov-Smirnov tests to determine whether treatment calibrations are significantly different from their respective base calibrations. All files are .csv, .txt, .Rmd, or .pdf.

本数据包与投稿至《公共科学图书馆·综合》（PlosONE）的论文《代谢多反应器：用于高通量分批反应器代谢实验的简易氧传感光极（optodes）的实用考量》（Kaufman等人，2023；DOI:10.1101/2023.03.28.534656）相关。本研究针对专为环境问题研究中的水体与沉积物样本设计的小型耗氧分批反应器系统，开展了大量测试与校准实验。该氧传感系统直接基于Larsen等人[2011]的研究成果，同类氧传感技术在相关学术文献中已得到广泛应用。本研究的核心聚焦于各类实用考量，例如温度效应、光照角度效应、灭菌操作，以及其他对使用者具有实用价值的相关场景。多数测试需将“基准”校准曲线与“处理组”校准曲线进行比对，以明确处理方式对报告测量结果的影响程度。本数据包包含为实现上述研究目标所采集的性能数据与校准数据。本数据集由单个数据文件夹构成，内含以下内容：(1) 文件级元数据；(2) 数据字典；(3) 自述文件（readme）；(4) 扩散测试结果文件；(5) 检测限测试结果文件；(6) 温度影响实验文件；(7) 涵盖其余所有测试结果的主数据文件；以及(8) 用于通过科尔莫戈罗夫-斯米尔诺夫检验（Kolmogorov-Smirnov tests）判定处理组校准曲线与对应基准校准曲线是否存在显著差异的R脚本。所有文件格式均为.csv、.txt、.Rmd或.pdf。