Analysis of RNA polymerase II phosphorylation in two-color STED microscopy images

This data set includes the raw image data and MatLab analysis scripts to assess the relative distribution of RNA polymerase II C-terminal domain serine 5 and serine 2 phosphorylation in zebrafish embryos. Phosphorylated polymerase II was labeled by immunofluorescence, microscopy images were acquired by STED microscopy and analyzed using MatLab scripts. This extended version now contains image data and analysis scripts to two alternative sets of antibodies.本数据集包含原始图像数据与MatLab分析脚本，用于评估斑马鱼胚胎中RNA聚合酶II（RNA polymerase II）C端结构域（C-terminal domain）丝氨酸5与丝氨酸2磷酸化的相对分布情况。经磷酸化修饰的RNA聚合酶II通过免疫荧光法完成标记，显微镜图像由受激发射损耗显微镜（STED microscopy）采集，并通过MatLab脚本完成分析。本扩展版本新增了针对两组替代抗体的图像数据及分析脚本。