Repeated evolution of photoperiodic plasticity by different genetic architectures during recurrent colonizations in a butterfly

In cases of recurrent colonization of similar habitats from the same base population, it is commonly expected that repeated phenotypic adaptation is caused by parallel changes in genetic variation. However, it is becoming increasingly clear that similar phenotypic variation may also evolve by alternative genetic pathways. Here we explore the repeated evolution of photoperiodic plasticity for diapause induction across Swedish populations of the Speckled wood butterfly Pararge aegeria. This species has colonized Scandinavia at least twice and population genomic results show that one of the candidate regions associated with spatial variation in photoperiodism is situated on the Z-chromosome. Here we assay hybrid crosses between several populations that differ in photoperiodic plasticity for sex-linked inheritance of the photoperiodic reaction norm. We find that while a cross between more distantly related populations from the two different colonization events shows strong sex-dependent inh..., The data is combined from the current experiment and the experiment from the article: Pruisscher, P., Nylin, S., Gotthard, K. and Wheat, C.W., 2018. Genetic variation underlying local adaptation of diapause induction along a cline in a butterfly. Molecular ecology, 27(18), pp.3613-3626. , , # Repeated evolution of photoperiodic plasticity by different genetic architectures during recurrent colonizations in a butterfly

[https://doi.org/10.5061/dryad.9zw3r22pw](https://doi.org/10.5061/dryad.9zw3r22pw)

## Description of the data and file structure

This file contains information about the data files used in the manuscript entitled “Repeated evolution of photoperiodic plasticity by different genetic architectures during recurrent colonizations in a butterfly”. Descriptions of the data and explanations for each column in the data tables are given under each file name below.

### Files and variables

#### File: means1\_LARVAL\_DECISION.csv

**Description:** The proportion of individuals inducing diapause in the larval decision within each treatment for every genotype (GG, SS, CC).

##### Variables

* pop: the genotype, either GG (Gotland), SS (Stockholm) or CC (the reciprocal crosses of GG and SS)
* treatm: the photoperiodic treatment of either 17, 17.4, 17.8, 18.2 or 18.6 hou...

当同一基础种群反复定殖相似生境时，学界通常认为重复的表型适应性由遗传变异的平行变化所驱动。然而越来越多的研究表明，相似的表型变异也可通过其他遗传路径演化而来。本研究以瑞典境内的斑木蝶（Speckled wood butterfly，学名*Pararge aegeria*）种群为研究对象，探讨滞育诱导（diapause induction）相关的光周期可塑性（photoperiodic plasticity）的重复演化历程。该物种至少两次定殖斯堪的纳维亚半岛，种群基因组学（population genomic）分析结果显示，与光周期响应的空间变异相关的候选区域之一位于Z染色体（Z-chromosome）上。本研究对多个光周期可塑性存在差异的种群进行杂交实验，以检测光周期反应规范（reaction norm）的伴性遗传（sex-linked inheritance）模式。我们发现，尽管来自两次不同定殖事件的亲缘关系较远的种群间杂交表现出强烈的性别依赖型遗传[原文此处截断]，本研究数据整合了本次实验与2018年发表的以下研究中的实验数据：Pruisscher, P., Nylin, S., Gotthard, K. 及 Wheat, C.W. 2018. 蝴蝶沿梯度分布的滞育诱导局部适应性的遗传基础. 《分子生态学》（*Molecular Ecology*）, 27(18), 第3613-3626页。

# 蝴蝶反复定殖过程中不同遗传架构下光周期可塑性的重复演化
https://doi.org/10.5061/dryad.9zw3r22pw

## 数据与文件结构说明
本文件包含题为《蝴蝶反复定殖过程中不同遗传架构下光周期可塑性的重复演化》的手稿中所用数据文件的相关信息。各数据表格的列项说明与数据描述将在下方各文件名下列出。

### 文件与变量
#### 文件：means1_LARVAL_DECISION.csv
**描述**：各处理组内，不同基因型（GG、SS、CC）个体的幼虫滞育决策诱导比例。
##### 变量说明
* pop：基因型，取值为GG（哥得兰岛种群）、SS（斯德哥尔摩种群）或CC（GG与SS的正反交组合）
* treatm：光周期处理时长，取值为17、17.4、17.8、18.2或18.6小时……