New spontaneous microphthalmia and aphakia (miak) mutations in mice.

A–D. The visible phenotypes of the KOR1/Stm (A) and KOR1/Stm-miak/miak homozygous mouse (B). Comparison of the histological phenotypes between the wild-type (C) and miak/miak (D) mice at 6 weeks of age in the KOR1/Stm genetic background. The miak mutant mouse shows small eyes without severely closed eyelids (B). A lens section of the miak mouse shows the large fraction of crystallin lens (*) with a tissue-like lens capsule (arrowhead) not present in the eye (D). co, cornea; L, lens; ir, iris; re, retina. Scale bar = 100 µm. E–H. Gross appearance (E, F) and histological features (G, H) of the miak/+ heterozygous (E, G) and miak/miak homozygous (F, H) mouse in the C57BL/6J background. In the miak/miak mice, the eye phenotypes are identical in the KOR1/Stm (B, D) and C57BL/6J (F, H) backgrounds. I. Quantitative analysis of the eye axial length in wild-type (+/+) and miak/miak mice in KOR1/Stm and +/+, miak/+ and miak/miak in C57BL/6J background at 6 weeks of age. ***P