CHLOROPHYLL A CONCENTRATION collected from NOAA Ship OSCAR ELTON SETTE in Hawaii EEZ and Palmyra EEZ from 2011-10-20 to 2011-11-17 (NCEI Accession 0155948)

Water samples were collected from the ocean surface using a bucket and from below the surface using bottles attached to the CTD during a Pacific Islands Fisheries Science Center's Cetacean Research Program's shipboard cetacean survey (Cruise ID: SE 11-08). A minimum of three surface water samples were taken each day, primarily at 0900, 1200, and 1500 hours local ship time. Surface water samples were also collected opportunistically during some cetacean sightings. CTD samples were collected once each morning.

The 250ml water samples were filtered onto GF/F filters, placed in 10ml of 90% acetone, refrigerated or frozen for 24 hours, and then analyzed for chlorophyll a concentration using the Turner Designs model 10AU field flourometer. Measurements were recorded in an Excel spreadsheet.

本次海水样品采集工作依托太平洋岛屿渔业科学中心（Pacific Islands Fisheries Science Center）鲸类研究项目的船载鲸类调查航次（航次编号：SE 11-08）开展。表层海水样品采用水桶采集，水下样品则通过连接于温盐深仪（CTD，Conductivity-Temperature-Depth）的采水瓶获取。每日至少采集3份表层海水样品，采样时段主要为当地船时09:00、12:00及15:00；部分鲸类目击作业期间，也会临时采集表层海水样品。每日晨间采集1次CTD采水样品。
将250mL海水样品过滤至GF/F玻璃微纤维滤膜（GF/F glass microfiber filter）上，随后将滤膜置于10mL 90%丙酮溶液中，经24小时冷藏或冷冻处理后，使用特纳设计10AU型野外荧光计（Turner Designs Model 10AU field fluorometer）测定叶绿素a浓度。所有测定数据均记录于Excel电子表格中。