IRF4 Haploinsufficiency Impairs Affinity Maturation

Single cell RNA seq (scRNA-seq) has emerged as a powerful tool to determine the composition of heterogeneous cell states in a tissue. We found that Irf4+/- antigen specific B cells were functionally impaired in affinity maturation. The goal of this study was to determine whether Irf4+/- antigen specific B cells exhibited distinct cellular composition compared to wild type cells. Sequence data from 8360 cells revealed a similar distribution and numbers of cell states between cells of Irf4+/- or Irf4+/+ genotypes. Overall design: scRNA-seq of Irf4+/- and Irf4+/+ antigen specific Germinal Center B cells at day 10 of an immune response.