Evolutionary repair: changes in multiple functional modules allow meiotic cohesin to support mitosis

This project contains whole-genome sequencing data of evolved budding yeast populations in Hsieh et al (Evolutionary repair: changes in multiple functional modules allow meiotic cohesin to support mitosis). Budding yeast cells were forced to use a meiotic cohesin protein, Rec8, in mitosis. Compared to the Rec8-expressing ancestor, evolved populations increased their fitness over 1750 generations. We sequenced the genomes of ancestral strains and evolved populations at generation 375 and 1750 to identify putative adaptive mutations. This evolution experiment allows us to examine to what extent mutations in Rec8 itself or in other proteins sufficiently improve the role of Rec8 in mitotic chromosome segregation. We found all adaptive mutations are not in Rec8, but in other proteins directly or indirectly interacting with cohesin. This result suggests that the evolution of a new biological function that a protein performs depends on other functionally interacting partners, rather than the protein itself.