\"Identification and assessment of single nucleotide polymorphisms (SNPs) between Culex complex mosquitoes.\" in Genomic Resources Notes Accepted 1 August 2014-30 September 2014

Culex pipiens complex mosquitoes are important vectors for many human pathogens including West Nile encephalitis, Rift Valley fever and Lymphatic filariasis. In this study we characterized a set of SNP markers between two biotypes of the Culex pipiens complex, Culex pipiens form molestus and Culex pipiens form pipiens, for use in a high-resolution genetic mapping and population genetics. DNA pooled from 10 specimens of each biotype were sequenced and analyzed for variation in 28 genes. The total of 4714 bp across orthologs of C. pipiens form pipiens and C. pipiens form molestus revealed 44 SNPs in the 3279 bp coding regions, 48 SNPs in the 1435 bp non-coding regions, and 10 indels. The ratio of transitions to transversions approached 2:1, with transitions constituting the majority of synonymous coding substitutions. The informative SNP markers were successfully identified and assessed from both C. pipiens biotypes. We expect that novel SNPs characterized in this study would be useful fo...

尖音库蚊复合组（Culex pipiens complex）蚊子是多种人类病原体的重要传播媒介，其传播的病原体包括西尼罗脑炎、裂谷热与淋巴丝虫病。本研究针对尖音库蚊复合组的两个生物型——尖音库蚊指名型（Culex pipiens form pipiens）与骚扰库蚊型（Culex pipiens form molestus），表征了一组单核苷酸多态性（Single Nucleotide Polymorphism, SNP）标记，以用于高分辨率遗传作图与群体遗传学研究。研究人员将每个生物型的10份标本的DNA混合后进行测序，并对28个基因的变异情况展开分析。在尖音库蚊指名型与骚扰库蚊型的同源基因序列中，总长4714 bp的区域共检测到变异位点：其中3279 bp的编码区内存在44个SNP位点，1435 bp的非编码区内存在48个SNP位点，另有10个插入缺失位点（insertion-deletion, indel）。转换与颠换的比例接近2:1，同义编码替换中的绝大多数为转换类型。本研究成功从两种尖音库蚊生物型中鉴定并评估了具备信息价值的SNP标记。我们预期本研究中表征的新型SNP标记将具有重要应用价值……