miRNA-seq of CD4+ T cells transfected by ASO or CRO

During the activation of CD4+T cells, the pri-miRNAs translocated from cytoplasma into nucleus.However, the mechanism remains unkown. We found that pri-miR-31 interact with nucleolin through G-guadruplex struction in pri-miR-31. We then transfected ASO to destribute the interaction of nucleolin and G4 struction. miRNA-seq was performed in our study. Overall design: ASO and its control CRO was transfected into naïve CD4+ T cells. The transfected cells were activated by anti-cd3/28. After 48 hours, cells were collected and went for RNA extraction. miRNA-seq was then ferformed.