BXSB_mice_IgG_glycosylation_batch1.1

Batch 1. Part 1.

The folder Batch1.1 contains mzXML files obtained in a liquid chromatography-mass spectrometry (LC-MS) analysis of Fc-linked IgG N-glycosylation profiles of mouse immunoglobulin G in a BXSB mice cohort, first part of batch1.
Second part of batch1 can be found in the following dataset: http://dx.doi.org/10.17632/kjfw7cwnxf.1
Batch 2 can be found in the following dataset: http://dx.doi.org/10.17632/4s9nxvk7br.1
Male BXSB mice spontaneously develop an autoimmune disorder similar to systemic lupus erethematousus (SLE) which involves production of autoreactive antibodies.
We have isolated IgG from serum sampled at 10 and 18 weeks of age in a cohort of sex-matched BXSB mice, treated either with anti-CD20 specific antibody that transiently depletes B-cells during the pre-phase of the disease or with an isotype antibody (control). IgG was digested with trypsin, the glycopeptides desalted and enriched by reverse-phase solid phase extraction and analyzed on an LC-MS system.

We observed higher abundance of sialylated IgG1 and IgG2b/IgG2c glycoforms in the male mice treated with CD20-specific antibody as compared to isotype-treated male mice, while no major changes were observed in galactosylated IgG glycoforms between isotype and CD20-specific antibody treated male mice.

Information about glycoforms observed in the samples and corresponding m/z values of analytes can be found in the excel sheet: Supplementary Table S1.xlsx
Description of the samples could be found in the following excel sheet: Supplementary Table S2.xlsx

批次1的第1部分。
文件夹Batch1.1中包含的mzXML文件，来源于对BXSB小鼠队列中小鼠免疫球蛋白G（immunoglobulin G, IgG）的Fc连接型IgG N-糖基化谱开展的液相色谱-质谱（liquid chromatography-mass spectrometry, LC-MS）分析，为本批次1的第一部分内容。

批次1的第二部分可在以下数据集获取：http://dx.doi.org/10.17632/kjfw7cwnxf.1
批次2可在以下数据集获取：http://dx.doi.org/10.17632/4s9nxvk7br.1

雄性BXSB小鼠可自发形成与系统性红斑狼疮（systemic lupus erythematosus, SLE）相似的自身免疫性疾病，该疾病会产生自身反应性抗体。

本研究从按性别匹配的BXSB小鼠队列中，采集10周龄与18周龄的血清以分离IgG。该队列小鼠分别接受两种处理：其一为靶向CD20的特异性抗体（可在疾病前驱期暂时性耗竭B细胞），其二为同型抗体（作为对照组）。将IgG经胰蛋白酶消化后，对糖肽进行脱盐并通过反相固相萃取完成富集，随后通过LC-MS系统进行分析。

本研究观察到：与经同型抗体处理的雄性小鼠相比，经CD20特异性抗体处理的雄性小鼠体内，唾液酸化IgG1与IgG2b/IgG2c糖型的丰度更高；而在两类抗体处理的雄性小鼠之间，半乳糖基化IgG糖型未出现显著变化。

样品中检测到的糖型信息及对应分析物的质荷比（m/z）可参见Excel表格Supplementary Table S1.xlsx；样品的相关描述可参见Excel表格Supplementary Table S2.xlsx。