Raw data supporting: D-Enantiomeric β-Casein 197 Disrupts Biofilm-Associated Infection of MDR Pseudomonas aeruginosa and Acinetobacter baumannii through Superior LPS Binding and Host Cell Protection

This dataset contains all raw data supporting the findings reported in the manuscript: "D-Enantiomeric β-Casein 197 Disrupts Biofilm-Associated Infection of MDR Pseudomonas aeruginosa and Acinetobacter baumannii through Superior LPS Binding and Host Cell Protection."File descriptions:Biofilm_RawData.xlsx — Raw OD570 values from crystal violet biofilm inhibition and eradication assays against four MDR P. aeruginosa (R1-R4) and four MDR A. baumannii (13-16) clinical isolates (n=5 per group).Cell viability_RawData.xlsx — Raw CCK-8 assay OD450 values for cytotoxicity evaluation of L- and D-β-casein 197 against A549 and J774A.1 cells at concentrations of 12.5, 25, 50, and 100 μM (n=3).FlowCyto_RawData.xlsx — Quadrant population percentages (Q1-Q4) from SYTO9/PI flow cytometry membrane integrity assays against all tested MDR isolates (n=3 per group).Flow_Cytometry_Raw_Plots.pdf — Representative raw dot plots from flow cytometry analysis showing gating strategy and quadrant populations.Galleria_RawData_Figshare.xlsx — Individual larval survival data from Galleria mellonella infection model (n=15 per group, 96 h observation, MDR P. aeruginosa R1 and A. baumannii 13).ITC_RawData.xlsx — Raw isothermal titration calorimetry data (heat per injection, normalized heat, molar ratio) from three independent replicates of L- and D-β-casein 197 binding to LPS.LDH_CFU_RawData.xlsx — Raw OD490 values from LDH release assay and CFU counts from bacterial internalization assay in J774A.1 macrophages infected with MDR P. aeruginosa and A. baumannii (n=3 per group).TEM — Transmission electron microscopy original images of untreated, L-form-treated, and D-form-treated MDR P. aeruginosa and A. baumannii cells.Biofilm CLSM.zip — Original confocal laser scanning microscopy images from biofilm inhibition and eradication assays stained with SYTO9/PI.All data are released under CC BY 4.0 licence.本数据集包含支撑发表于论文《D-对映异构体β-酪蛋白197通过优异的脂多糖（Lipopolysaccharide, LPS）结合能力与宿主细胞保护作用破坏多重耐药（Multidrug Resistant, MDR）铜绿假单胞菌与鲍曼不动杆菌的生物膜相关感染》的全部原始实验数据。文件详情如下： - Biofilm_RawData.xlsx：针对4株多重耐药铜绿假单胞菌（R1-R4）与4株多重耐药鲍曼不动杆菌（13-16）临床分离株的结晶紫生物膜抑制与清除实验的原始OD570吸光度值（每组n=5）。 - Cell viability_RawData.xlsx：针对浓度为12.5、25、50与100 μM的L-与D-β-酪蛋白197，采用CCK-8实验检测其对A549与J774A.1细胞的细胞毒性的原始OD450吸光度值（每组n=3）。 - FlowCyto_RawData.xlsx：针对所有受试多重耐药菌株的SYTO9/PI流式细胞术细胞膜完整性实验的象限（Q1-Q4）细胞群体占比数据（每组n=3）。 - Flow_Cytometry_Raw_Plots.pdf：流式细胞术分析的代表性原始散点图，包含门控策略与象限群体分布。 - Galleria_RawData_Figshare.xlsx：大蜡螟（Galleria mellonella）感染模型的幼虫个体存活数据（每组n=15，观察时长96小时，受试菌株为多重耐药铜绿假单胞菌R1与鲍曼不动杆菌13）。 - ITC_RawData.xlsx：L-与D-β-酪蛋白197与脂多糖结合实验的三次独立重复的原始等温滴定量热（Isothermal Titration Calorimetry, ITC）数据（涵盖每注射热流、归一化热流与摩尔比）。 - LDH_CFU_RawData.xlsx：以J774A.1巨噬细胞感染多重耐药铜绿假单胞菌与鲍曼不动杆菌后，乳酸脱氢酶（Lactate Dehydrogenase, LDH）释放实验的原始OD490吸光度值与细菌内化实验的菌落形成单位（Colony Forming Unit, CFU）计数数据（每组n=3）。 - TEM：未处理组、L-型处理组与D-型处理组的多重耐药铜绿假单胞菌与鲍曼不动杆菌细胞的透射电子显微镜（Transmission Electron Microscopy, TEM）原始图像。 - Biofilm CLSM.zip：经SYTO9/PI染色的生物膜抑制与清除实验的原始共聚焦激光扫描显微镜（Confocal Laser Scanning Microscopy, CLSM）图像压缩包。 所有数据均采用CC BY 4.0协议发布。