Transcriptomics of chemically induced hepatocyte-like cells from mouse fibroblasts [RNA-seq]

Here, we chemically induced hepatocyte-like cells (CiHeps) from mouse fibroblasts using a new protocol. To compare the transcriptional profiles between different cocktail-induced cells and primary hepatocytes (pHeps), we profiled the transcriptomes of induced cells and freshly isolated primary mouse hepatocytes. CiHeps' function and maturation-related gene expression was validated as well as the similarity it shares with pHeps. Comparative gene expression profiling analysis of RNA-seq data for Mouse embryonic fibroblasts (MEFs), MEFs induced with chemical cocktails, including C6FAE, C6F(L), C6FAE-C6F(L), C6F5UE-C6F(L)V, NSF-derived CiHeps (induced by C6F5UE-C6F(L)V), or pHeps.