Recombinant mouse CRISP protein add-back experiments

I. Representative sperm from Crisp1, and Crisp4 wild type and knock out mice before and after exposure to 1 uM recombinant mouse CRISP proteins. II. Representative sperm from Crisp1 knockout sperm before and after exposure to 1 uM recombinant mouse CRISP4 protein and vice versa.Sperm were tethered to glass slides and recorded using an Olympus AX-70 upright microscope equipped with ORCA-Flash4.0 v2 sCMOS camera. Frames were acquired at 400 fps.I. 取自Crisp1、Crisp4野生型与基因敲除（knock out）小鼠的代表性精子，在暴露于1 μM重组小鼠CRISP蛋白前后的样本。 II. 取自Crisp1基因敲除小鼠的代表性精子，在暴露于1 μM重组小鼠CRISP4蛋白前后的样本，以及反之（即取自Crisp4基因敲除小鼠的代表性精子，在暴露于1 μM重组小鼠CRISP1蛋白前后的样本）。 将精子固定于载玻片上，使用搭载ORCA-Flash4.0 v2 sCMOS相机的Olympus AX-70正置显微镜进行录制。图像帧以400 fps的帧率采集。