Transcription profiling by array of human A375 cells transfected with Neg-miRNA, HSPE-miRNA1 and HSPE-miRNA2 to study to explore the probable mechanism of HPSE-miRNA on melanoma metastasis

Heparanase (HPSE) is an endo-beta-glucuronidase that can cleave heparan sulfate proteoglycans within extracellular matrix, basement membrane or on the cellular surface, closely linked with proliferation, migration, invasion and angiogenesis of tumor cells, and has become a hot treatment target in anti-cancer metastasis. In order to explore the probable mechanism of HPSE-miRNA on melanoma metastasis and to distinguish whether the silence effect on unpredicted genes was due to off-target effect of RNAi, we analyzed and compared genes between the Neg-miRNA transfected A375 cells and HPSE-miRNA1 or HPSE-miRNA2 transfected A375 cells by gene microarray analysis using OneArray(r) slides.