Footprinting SHAPE-eCLIP reveals transcriptome-wide hydrogen bonds at RNA-protein interfaces. Corley et al.

EMSAs testing binding of candidate iron response elements by iron response proteins, and RT-qPCR measuring transcript level response of these candidates to various cellular iron conditions.

本数据集包含两类实验数据：一是通过电泳迁移率变动分析（Electrophoretic Mobility Shift Assay, EMSAs）检测铁应答蛋白（iron response proteins, IRPs）与候选铁应答元件（iron response elements, IREs）结合活性的实验结果；二是利用实时定量逆转录聚合酶链反应（Reverse Transcription Quantitative Polymerase Chain Reaction, RT-qPCR）测定上述候选元件在不同细胞铁状态下转录本水平应答情况的实验结果。