<i>Drosophila</i> Actograms of Locomotor Behavior.

Wild-type Iso31 flies entrained to a 12∶12 hour light (yellow)∶dark (gray) schedule display evening peak activity in pressurized air control (black) and isoflurane (purple) groups. One day prior to gas exposure, activity patterns are similar in the (A) air control group and (B) isoflurane group. On the experimental day, the duration of exposure to pressurized air with or without anesthetic gas is indicated by the thin horizontal black bar. (C) Activity pattern remains unchanged in the air control group. (D) Induction of anesthesia is marked by an abrupt cessation of activity in the isoflurane group, while emergence is marked by the resumption of activity. On the day following gas exposure, normal evening activity patterns are observed for flies previously exposed to (E) pressurized air without any anesthetic and (F) pressurized air containing isoflurane.