High‑fidelity genome assembly of wheat introgression line Zahir‑1644

This dataset provides a high‑fidelity genome assembly of the wheat introgression line Zahir‑1644, generated using PacBio Sequel II Hi‑Fi reads and assembled with hifiasm (v0.19). The assembly, which has a contig N50 of 21 Mb, was quality‐assessed using QUAST (v5.2) and BUSCO (v5.1) against the ‘poales’ lineage, demonstrating high completeness and contiguity. These data support studies on wheat disease resistance mechanisms and genetic improvement. Included files comprise the raw sequencing reads, the assembled genome in FASTA format, and associated assembly metrics. Methods Genomic DNA was extracted from the leaf tissues of Zahir-1644 introgressed line and sequenced through PacBio Hi-Fi sequencing platform. The raw reads were processed and then assembled using Hifiasm genome assembler.