Crystal structure of Paradendryphiella salina PL7A alginate lyase mutant Y223F in complex with tetra-mannuronic acid

Crystal structure of Paradendryphiella salina PL7A alginate lyase mutant Y223F in complex with tetra-mannuronic acid Descriptor: 2-[BIS-(2-HYDROXY-ETHYL)-AMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, Alginate lyase (PL7), beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid-(1-4)-beta-D-mannopyranuronic acid Authors: Fredslund, F, Welner, D.W, Wilkens, C. Deposit date: 2021-03-20 Release date: 2022-03-30 Last modified: 2025-07-02 Method: X-RAY DIFFRACTION (1.82 Å) Cite: Unraveling the molecular mechanism of polysaccharide lyases for efficient alginate degradation. Nat Commun, 16, 2025

本数据集记录盐生副丹毒霉（Paradendryphiella salina）PL7A褐藻胶裂解酶（alginate lyase, PL7）突变体Y223F与四聚甘露糖醛酸复合物的晶体结构。详细信息如下：成分描述：2-[双(2-羟乙基)氨基]-2-(羟甲基)丙烷-1,3-二醇、褐藻胶裂解酶（alginate lyase, PL7）、β-D-吡喃甘露糖醛酸-(1→4)-β-D-吡喃甘露糖醛酸-(1→4)-β-D-吡喃甘露糖醛酸-(1→4)-β-D-吡喃甘露糖醛酸-(1→4)-β-D-吡喃甘露糖醛酸-(1→4)-β-D-吡喃甘露糖醛酸；作者：Fredslund, F、Welner, D.W、Wilkens, C.；沉积日期：2021年3月20日；发布日期：2022年3月30日；最后修改日期：2025年7月2日；实验方法：X射线衍射（X-RAY DIFFRACTION），分辨率1.82埃（Å）；引用文献：原标题为《Unraveling the molecular mechanism of polysaccharide lyases for efficient alginate degradation》，中文译名为《解析多糖裂解酶高效降解褐藻胶的分子机制》，发表于《Nature Communications》（《自然·通讯》）2025年第16卷。