Period wavelet movies of spreadouts, ex vivo models of somitogenesis, subjected to 170-min periodic pulses of 2 uM DAPT

Here are period wavelet movies (as .tif), generated using a wavelet analysis workflow developed by Gregor Mönke, of timelapse imaging of a dynamic Notch signaling reporter (i.e. LuVeLu) in spreadouts, ex vivo models of somitogenesis. Spreadouts were subjected to 170-min periodic pulses of 2 uM DAPT. Also here are binary masks generated in Fiji based on threshold of LuVeLu intensity. These files are used to run an accompanying Python script, available at: https://github.com/PGLSanchez/EMBL_OscillationsAnalysis/tree/master/PeriodGradientAnalysis本数据集包含由Gregor Mönke开发的小波分析流程生成的周期小波影像（格式为.tif），这些影像对应体节发生离体模型铺展标本中动态Notch信号报告基因（即LuVeLu）的延时成像结果。该铺展标本接受了浓度为2 μM的DAPT的170分钟周期性脉冲处理。此外还包含通过Fiji软件基于LuVeLu信号强度阈值生成的二值掩膜文件。上述文件可用于运行配套的Python脚本，该脚本的开源地址为：https://github.com/PGLSanchez/EMBL_OscillationsAnalysis/tree/master/PeriodGradientAnalysis