Global analysis of dauer gene expression in Caenorhabditis elegans

These are the 94 microarray experiments that are published in the paper: John Wang and Stuart K. Kim. Global analysis of dauer gene expression in Caenorhabditis elegans, Development 2003 130: 1621-1634. There are 94 individual microarray experiments divided into 3 broad experiments. The first experiment is a time course of dauer exit; each time course is labeled as "Dauer MTC#". The second experiment is a time course of L1 development after starvation arrest; each time couse is labeled "L1 MTC#". The final experiment is a comparison of pure dauers (0 hours) versus 12 hours after dauer exit and are labeled "Dauer Adjust". Every time course was repeated 4 times (#N)however for the dauer 4 and 7 hour time points there are only 3 replicates. For instance, all the time points labeled as "Dauer MTC#1" are from the same starting pool of dauer worms that were aliquoted into 10 fractions and analyzed at the time indicated. Every sample is compared to a common reference RNA that is used throughout all the hybridizations. In some cases there is a "-2" after the hour designation; this means the first hybridization failed for some technical reason and thus the second hybridization (same RNA) is reported. Groups of assays that are related as part of a time series. Keywords: time_series_design本数据集包含94项微阵列实验，全部源自John Wang与Stuart K. Kim发表于期刊《Development》2003年第130卷第1621-1634页的研究论文《Global analysis of dauer gene expression in Caenorhabditis elegans》（秀丽隐杆线虫（Caenorhabditis elegans）dauer态（dauer）基因表达的全局分析）。该94项独立微阵列实验可划分为3大类实验：第一类实验为dauer态退出的时间进程实验，所有该类时间进程样本均标记为"Dauer MTC#"；第二类实验为饥饿阻滞后L1期发育的时间进程实验，该类时间进程样本标记为"L1 MTC#"；第三类实验为纯dauer态样本（0小时）与dauer态退出12小时样本的对照实验，标记为"Dauer Adjust"。所有时间进程实验均完成4次重复（记为#N），但dauer态4小时与7小时的时间点仅设有3次重复。例如，所有标记为"Dauer MTC#1"的时间点样本均取自同一批起始dauer线虫种群，该种群被均分至10个组分，并按指定时间点完成检测分析。每一份待测样本均与所有杂交实验中统一使用的参考RNA进行比对。部分样本的时间标识后带有"-2"，这代表首次杂交因技术故障失败，因此最终报告的是同一份RNA的第二次杂交结果。本数据集包含一组按时间序列设计的关联检测实验。关键词：时间序列设计