Effects of RPAP1 depletion on mRNA gene expression (RNA-seq) and RNA Pol II abundance (ChIP-seq) in primary mouse embryonic fibroblasts or embryonic stem cells.
收藏干细胞与再生医学数据中心2022-02-20 更新2024-03-06 收录
下载链接：
http://data.iscr.ac.cn/Article?id=c535931c0bb683fc683649b9b86d65ff
下载链接
链接失效反馈官方服务：
资源简介：
Here, we investigated the role of RPAP1 in mammalian cell identity. As in plants, interfering with RPAP1 function did not affect the viability of mouse embryonic stem cells, but severely impaired their differentiation capacity (RNA-Seq#2 data, below). Conversely, in somatic cells, RPAP1 was essential for the expression of lineage specifying factors and viability (RNA-seq#1 data, below). Moreover, inhibition of RPAP1 triggers dedifferentiation and facilitates reprogramming into pluripotent stem cells. Mechanistically, RPAP1 maintains transcribing Pol II levels and Pol II Ser5 phosphorylation, particularly on developmental genes (ChIP-seq data, below).
提供机构：
Spanish National Cancer Research Centre (CNIO)
创建时间：
2022-02-20
