Quantifying yeast lipidomics by high performance thin layer chromatography (HPTLC) and comparison to mass spectrometry-based shotgun lipidomics.

Here we present evidence that high performance thin layer chromatography (HPTLC) represents an effective alternative to replace cost intensive mass spectrometry-based lipidomic analyses. We provide statistical comparison of identical samples by both methods, which support the use of HPTLC for quantitative analysis of the main yeast lipid classes.

本研究提供证据表明，高效薄层色谱（high performance thin layer chromatography, HPTLC）可作为有效替代方案，取代成本高昂的基于质谱的脂质组学分析。本研究针对同一批样本采用两种方法开展统计学对比，结果证实HPTLC可用于酵母主要脂质类别的定量分析。