Single Vs. Pooled: Metabarcoding based species misrepresentation detection via sample pooling for efficacious traceability of sushi in Canada: Key methodological uncertainties compared to Conventional DNA Barcoding

This data set contains the results of the Sanger Sequencing based DNA barcoding seafood tissue samples from sushi sold in grocery stores and sushi restaurants across Ontario, Canada. It also contains the results of the metabarcoding of the sushi samples when pooled into 21 sample pools with each sample pool containing 2-20 seafood tissue samples from sushi, detected by Sanger sequencing analysis above. The Sanger sequencing was carried out using hierarchical primer amplification approach using universal Fish COI primer as key primer and 12S and 16S based primers as alternatives. Metabarcoding of each primer pool was carried out using five primers, MiTuna, MiFish, MiDeca_MiOcto, SSU and Mini_SH_D and Mini_SH_E primers. Five control sample pools, C1-C5 were also tested, in addition (2021-12-01).

本数据集包含基于桑格测序（Sanger Sequencing）的DNA条形码（DNA barcoding）分析结果，样本取自加拿大安大略省各地杂货店及寿司餐厅售卖的寿司中的海鲜组织样品。本数据集同时涵盖寿司样品的宏条形码（metabarcoding）分析结果：将寿司样品整合为21个样本混合池，每个混合池包含2~20份寿司海鲜组织样品，上述样品均通过前述桑格测序分析完成检测。本次桑格测序采用层级引物扩增策略，以通用鱼类COI引物作为核心引物，以基于12S和16S的引物作为备选引物。针对每个引物混合池的宏条形码测序，共使用5种引物，分别为MiTuna、MiFish、MiDeca_MiOcto、SSU、Mini_SH_D及Mini_SH_E引物。此外，本研究还对5个对照样本混合池（C1~C5）进行了检测，相关实验完成于2021年12月1日。