Abid lab research data for article named: Optimization of mito-roGFP protocol to measure mitochondrial oxidative status in human coronary artery endothelial cells

This dataset provides useful information for researchers planning to standardize the mito-roGFP protocol in their labs. To better understand this data, we suggest using the notebook notes as a guide to visualize the original .nd2 file and excel graph report. Alternatively, a summary of graph reports for each day and sample is provided as a .pptx file and can be visualized along with the notebook notes for a clear understanding of hydrogen peroxide optimization procedures. This data was obtained from a culture of HCAEC at passage 3. Note: .nd2 files can be visualized with the free version of NIS Elements software.

本数据集可为计划在实验室中标准化mito-roGFP实验方案的研究人员提供实用参考信息。为便于理解本数据集内容，我们建议借助配套笔记本注释文档作为指南，对原始.nd2格式文件与Excel图表报告进行可视化处理。此外，本数据集还提供了按每日及样本分组的图表报告摘要，以.pptx格式文件存储，可与笔记本注释文档配合查看，以清晰掌握过氧化氢优化实验的操作流程。本数据集的数据取自第3代人冠状动脉内皮细胞（HCAEC）的培养物。注意：.nd2格式文件可通过免费版NIS Elements软件完成可视化查看。