SWATH-MS Proteomics Analysis of Persicaria minor herb upon Methyl Jasmonate elicitation

Stresses from either biotic or abiotic origins can have significant impact towards plant physiology and molecular regulation. Jasmonate acid (JA) and its derivative, methyl JA (MeJA) are hormonal cues released by plants which signal defensive response to curb the damage from such stresses. In an attempt to study the defensive response, a tropical herbal plant, Persicaria minor (P. minor) which is known for its pungent smell as well as various bioactivities including antimicrobial and anti-cancer, has been treated with MeJA to invoke the stress signaling. Such elicitation has been performed in various plants such as Arabidopsis, rice and hairy root cultures of certain herbs, yet how MeJA directly influenced the proteome of a herbal species particularly P. minor has not been previously elucidated. In this study, P. minor plants was exogenously treated with MeJA and its proteome was investigated using a new proteomics approach called SWATH-MS.