Water Quality Data (Extensive) from the Taylor Slough, Everglades National Park (FCE), from April 1996 to Present

Water quality samples are being collected using ISCO autosamplers at all wetland sites (that is, all sites except TS/Ph-9, 10, and 11). The autosamplers contain 24 1L bottles. Water is sampled by programming the autosamplers to take composite samples once every 3 days. These samples are a composite of four 250mL subsamples drawn every 18 hours (a sampling scheme that captures a dawn, noon, dusk, and midnight sample in every three day composite). The samples are collected every 3-4 weeks and analyzed for total phosphorus (TP), total nitrogen (TN), and salinity. When sites are visited to collect these samples, we also collect a grab sample that is immediately put on ice. A portion of these grab samples is filtered through a Whatman GF/F filter immediately upon return to the lab, and the filtered samples are analyzed for inorganic nutrients such as NO2-, NO3-, NH4+, SRP, and DOC. The unfiltered fraction of these grab samples is analyzed for TP, TN, and TOC. We use these monthly grab samples to generate relationships between TP and SRP, and between TN and NO2- + NO3- + NH4+. Dissolved nutrients are measured using standard rapid flow analyzer (RFA) techniques. TP is analyzed with a modified Solorzano and Sharp (1980) technique. TN is measured with an Antec TN analyzer, TOC and DOC are quantified on a Shimadzu TOC Analyzer, and salinity is measured with an YSI conductivity meter. In addition to the regular water quality monitoring, we use the rain level actuators at all freshwater sites to trigger water sampling after rain events exceed a given threshold of duration and/or intensity. As currently programmed, when the threshold of = 2.5 cm of rain per hour is passed, the autosampler at that site collects a 500mL sample 30 minutes after the threshold has been reached. Rain event samples are collected, treated, and analyzed as all other water quality samples.

研究团队采用ISCO自动采样器（ISCO autosamplers）对所有湿地样点开展水质样品采集工作，即除TS/Ph-9、10、11号样点外的全部站点。该自动采样器配备24个容积为1L的采样瓶。通过对自动采样器进行编程设置，使其每3天采集一次混合水样：每18小时采集一份250mL的子样品，每3天的混合水样由4份该子样品混合而成，该采样方案可覆盖黎明、正午、黄昏与午夜四个时段的水样。每3至4周开展一次样品收集，并对总磷（Total Phosphorus, TP）、总氮（Total Nitrogen, TN）及盐度开展检测分析。在赴样点采集上述水样的同时，研究团队还会采集瞬时水样（grab sample），并立即将其置于冰上保存。返回实验室后，研究团队即刻使用Whatman GF/F滤膜（Whatman GF/F filter）对部分瞬时水样进行过滤，过滤后的水样用于检测亚硝酸盐（NO₂⁻）、硝酸盐（NO₃⁻）、氨氮（NH₄⁺）、可溶性活性磷（SRP）及溶解性有机碳（DOC）等无机营养盐。未经过滤的瞬时水样分样则用于检测总磷（TP）、总氮（TN）及总有机碳（TOC）。研究团队利用这些月度瞬时水样，建立总磷（TP）与可溶性活性磷（SRP）之间、总氮（TN）与亚硝酸盐（NO₂⁻）+硝酸盐（NO₃⁻）+氨氮（NH₄⁺）之间的相关关系。溶解态营养盐采用标准快速流动分析仪（Rapid Flow Analyzer, RFA）技术进行检测。总磷（TP）检测采用改良的Solorzano与Sharp（1980）方法；总氮（TN）检测使用Antec总氮分析仪；总有机碳（TOC）与溶解性有机碳（DOC）采用岛津（Shimadzu）总有机碳分析仪进行定量；盐度则通过YSI电导仪（YSI conductivity meter）测定。除常规水质监测外，研究团队还在所有淡水样点安装降雨触发装置，当降雨时长和/或强度达到设定阈值时，启动水样采集。按照当前的编程设置，当每小时降雨量达到2.5cm的阈值时，对应站点的自动采样器将在阈值触发30分钟后采集一份500mL的水样。降雨事件水样的采集、处理与分析流程与其他常规水质样品完全一致。